Mapping of neurogenesis in human hippocampi across ages and different cognitive abilities using multiomic single-cell sequencing reveals distinct signatures between cognitive preservation and decline.
This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
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"If my kids need me, I will be off with them - no question. We coordinate so that key moments for our children are protected, while the company still has a steady hand on the wheel," says Johansson.
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